Uncovering new antibiofilm roseabols from marine Clonostachys rosea through structural and multiomics approaches.

Abstract

Through a combined approach, that involved the one-strain-many-compounds (OSMAC) culturing approach, metabolomic and genomic studies, and bioassay-guided purification we disclose the marine fungus Clonostachys rosea as producer of a family of peptaibols with new structural features. HRMS/MS, NMR, and advanced Marfey's analysis enabled the structural elucidation of two major compounds of the roseabol family, named roseabols B (1) and C (2). Molecular networking further revealed a highly diverse and microheterogeneous peptaibol profile. Biological evaluation highlighted the ability of the isolated peptaibols to inhibit biofilm formation against Escherichia coli and Candida albicans. Overall, these studies demonstrate the effectiveness of metabolomics-driven strategies in discovering novel natural products and identify marine-derived peptaibols as promising antibiofilm agents.

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