Structure and gene cluster of the O-antigen of Enterobacter cloacae G2369.

Abstract

The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2369 and studied by chemical methods, including component analyses and Smith degradation, along with 1H and 13C NMR spectroscopy. The following structure of the branched heptasaccharide repeating unit was established. The O-antigen gene cluster of Enterobacter cloacae G2369 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in a good agreement with the O-antigen structure.

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